Investigation into Antiepileptic Effect of Ganoderic Acid A and Its Mechanism in Seizure Rats Induced by Pentylenetetrazole

Figure 1

Workflow of the study. Rats were divided into 4 groups; three of them were administered (i.p.) to induce seizure; then, they were given treatment with placebo (normal group), placebo (epilepsy group), sodium valproate (epilepsy treated group), or GAA (epilepsy treated group), respectively. Electroencephalography (EGG); epileptic behavior was assessed, and morphological changes and apoptosis were tested. Protein expression of calcium-sensing receptor, p-ERK, p-JNK, p-p38, Bcl-2, cleaved caspase-3, and Bax in brain tissues was observed by Western blot and immunohistochemistry (IHC) assay, respectively.