Regulatory Effects of Fyn on Trophoblast Cell Behaviors and Function

Figure 4

Effects of Fyn on inflammatory cytokine production and HLA-G expression in trophoblast cells. (a–d) Cytokines, including TNF-α, IFN-γ, IL-4, and TGF-β, were measured by ELISA after Fyn knockdown, overexpression, or treatment with SU6656. (e) HLA-G protein expression in JEG-3 cells under various conditions was determined by western blotting. All experiments were repeated three times. vs. all other groups; # vs. the CON, shCtrl, OE vector, and OE Fyn groups; ^▲ vs. the CON, shCtrl, shFyn, and OE vector groups; ^△ vs. the OE vector and OE Fyn groups. shCtrl: control shRNA; shFyn: Fyn shRNA; OE vector: overexpression vector; OE Fyn: Fyn-overexpressing vector; iFyn: Fyn inhibitor, SU6656.

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