The Role of Methyl-(Z)-11-tetradecenoate Acid from the Bacterial Membrane Lipid Composition in Escherichia coli Antibiotic Resistance

Table 1

Sample preparation and reagents used.

Phase	Substances used
Saponification	Reagent 1: (i) 45 g sodium hydroxide; (ii) 150 mL methanol; (iii) 150 mL distilled water
Methylation	Reagent 2: (i) 325 mL clorhidric acid 6.0 N; (ii) 275 mL methanol
Extraction	Reagent 3: (i) 200 mL hexane; (ii) 200 mL methyl tert-butyl-ether (MTBE)
Rinse	Reagent 4: (i) 10.8 g sodium hydroxide; (ii) 900 mL distilled water