[Retracted] Expression Profile of MAGE-B1 Gene and Its Hypomethylation Activation in Colon Cancer

Figure 2

The effects of 5-aza-2-deoxycytidine on MAGE-B1 gene expression in the CC cell lines. MAGE-B1 gene expression was exhibited in 1% agarose gels following treatment with a variety of 5-aza-2-deoxycytidine doses (1.0, 5.0, and 10.0 μM) for 48 h (left side) or 72 hours (right side). Untreated HCT116 and Caco-2 cells were used for comparison with treated cells. Testis cDNA was used to validate the primer efficiency of the MAGE-B1 gene. HCT116 and Caco-2 cells were treated with DMSO as a control, as DMSO was utilized to prepare the 5-aza-2-deoxycytidine solution. As a positive control for the cDNA samples, ACTB expression was used. The expected size of each gene’s product is indicated on the left between brackets.