Anti-CD44 and EGFR Dual-Targeted Solid Lipid Nanoparticles for Delivery of Doxorubicin to Triple-Negative Breast Cancer Cell Line: Preparation, Statistical Optimization, and In Vitro Characterization

Table 6

In vitro transcription protocol.

Component	Volume
DNA template	1-2 μg
T7 reaction buffer	5 μl
2-F NTP mix	1.5 μl
RNA polymerase	0.5 μl
DEPC treated water	Up to 25 μl