Mechanical Stretch Promotes Macrophage Polarization and Inflammation via the RhoA-ROCK/NF-κB Pathway

Figure 1

Mechanical stretch promoted M1-type morphological changes in RAW264.7 macrophages. (a) Schematic of Flexcell-5000T applying mechanical stretch. (b) The morphological changes of RAW264.7 macrophages were observed after 5%, 10%, and 15% elongation mechanical stretch for 8 h. Control indicates cells were cultured at static condition (100x). (c) FITC-phalloidin immunofluorescence staining of cytoskeleton in RAW264.7 macrophages after 5%, 10%, and 15% Elong mechanical stretch for 8 h. Control indicates cells were cultured at static condition. DAPI detected nuclei. The white arrow points to the cell antenna. (400x).

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