Mechanical Stretch Promotes Macrophage Polarization and Inflammation via the RhoA-ROCK/NF-κB Pathway

Figure 6

Inhibition of ROCK1 signal can inactivate ROCK1/NF-κB pathway of MS-treated RAW264.7 macrophages. Protein expression of IL6, iNOS, Rho, ROCK1, ROCK2, NF-κB, Iκb-α, and p-Iκb-α was analyzed by Western blotting after 5%, 10%, and 15% Elong mechanical stretch for 8 h. Control indicates cells were cultured at static condition. The (+) Y27632 group was pretreated with Y27632 (10 μM, 12 h). Control indicates cells were cultured at static condition. Quantitative analysis of protein via the Image J software. The results are expressed as the (). Compare to control in MS, and . Compare to control in MS+Y-27632: ^# and ^##. Compare to MS group, and .