Ischemic Preconditioning and Postconditioning Protect the Heart by Preserving the Mitochondrial Network

Figure 3

IPC and IPost promotes mitochondrial elongation in cardiac cells. (a) Proportion of cells with elongated mitochondria following sIR, IPC-sIR, and sI-IPost-R (, ~ 70 cells/field); and (b) Representative confocal images of fragmented mitochondria (upper panel) and elongated mitochondria (lower panel) in H9c2 cells, observed using 100X oil immersion lens. (c) Average mitochondrial length in the different subpopulation of mitochondria (~2000 IFM/group, ~1000 PNM/group, ~1000 SSM/group; ). (d) Proportion of IFM with length of >2 μm in isolated primary adult primary cardiomyocytes ( mice; 60 cells/group; ~2000 IFM/group, ~1000 PNM/group, and ~1000 SSM/group). (e) Representative confocal images of mitochondrial morphology in isolated adult primary cardiomyocytes (left panel: elongated mitochondria; right panel: fragmented mitochondria).

(a)

(b)

(c)

(d)

(e)