Ischemic Preconditioning and Postconditioning Protect the Heart by Preserving the Mitochondrial Network

Table 1

Mitochondrial-shaping proteins primers for H9c2 cells.

Gene(s)	Primer’s sequence (5– 3)	Melting temperature (°C)	Product size (bp)
OPA1	Forward: CAGCTGGCAGAAGATCTCAAG; Reverse: CATGAGCAGGATTTTGACACC	58.72; 57.50	107
Mfn1	Forward: GGAGATACAGGGCTACAGAAAC; Reverse: AGCTCTTGCCACTACTTGTC	57.94; 57.54	104
Mfn2	Forward: TTGACTCCAGCCATGTCCAT; Reverse: GGTGACGATGGAGTTGCATC	59.01; 58.99	100
Drp1	Forward: TGGAGATGGTGGTCAGGAAC; Reverse: TTTCGTGCAACTGGAACTGG	59.02; 58.98	174
GAPDH	Forward: TGCCCCCATGTTTGTGATG; Reverse: GCTGACAATCTTGAGGGAGTTGT	58.33; 60.81	64