Ischemic Preconditioning and Postconditioning Protect the Heart by Preserving the Mitochondrial Network

Table 2

Mitochondrial-shaping proteins primers for primary cardiomyocytes.

Gene(s)	Primer’s sequence (5–3)	Melting temperature (°C)	Product size (bp)
OPA1	Forward: ACCTTGCCAGTTTAGCTCCC; Reverse: TTGGGACCTGCAGTGAAGAA	59.96; 59.16	82 bp
Mfn1	Forward: GATCCGATTCCGAGCTTCCG; Reverse: GATCCGATTCCGAGCTTCCG	59.75; 60.66	83 bp
Mfn2	Forward: TGCACCGCCATATAGAGGAAG; Reverse: TCTGCAGTGAACTGGCAATG	59.66; 58.76	78 bp
Drp1	Forward: ATGCCAGCAAGTCCACAGAA; Reverse: TGTTCTCGGGCAGACAGTTT	59.89; 59.53	86 bp
GAPDH	Forward: AGGTCGGTGTGAACGGATTTG; Reverse: TGTAGACCATGTAGTTGAGGT	60.88; 55.73	123 bp