BioMed Research International

Research Article

Antibiotic resistance’s Genotypic and Phenotypic Characteristics and the Frequency of Virulence Factors in P. aeruginosa Isolates Isolated from Water Samples in Iran

Table 1

List of primers used to detect virulence factors in P. aeruginosa strains isolated from water [28].
Virulence genesSequence (5–3)Size of product (bp)
algDF: AAGGCGGAAATGCCATCTCC
R: AGGGAAGTTCCGGGCGTTTG		275
algUF: CGCGAACCGCACCATCGCTC
R: GCCGCACGTCACGAGC		410
lasBF: ACACAATACATATCAACTTCGC
R: AGTGTGTTTAGAATGGTGATC		284
toxAF: ATGTGCAGYACCAGTAARGT
R: TGGGTRAARTARGTSACCAGA		270
plcHF: CACACGGAAGGTTAATTCTGA
R: CGGTTARACGGCTGAACCTG		608
plcNF: CGACTTCCATTTCCCGATGC
R: GGACTCTGCAACAAATACGC		481
exoSF: GTGTGCTTTATGCCATGAG
R: GGTTTCCTTTTCCAGGTC		444
exoTF: CATCGTCTACGCCATGAG
R: AGCAGCACCTCGGAATAG		1159
exoYF: GGAATGAACGAAGCGTTCTCCGAC
R: TGGCGTCGACGAACACCTCG		1035
exoUF: CTGCGCGGGTCTATGTGCC
R: GATGCTGGACGGGTCGAG		3308
aprF: GCACGTGGTCATCCTGATGC
R: TCCGTAGGCGTCGACGTAC		1017
phzIIF: TCCGTTATCGCAACCAGCCCTACG
R: TCGCTGTCGAGCAGGTCGAAC		1036
phzMF: CGTCGTGTTCAAGCAGATGGTGCTG
R: CCGAACCGCTTCACCAGGC		875
phzSF: CAATCATCTCAGCAGAACCC
R: TGTCGTAGAGGATCTCCTG		1752
phzIF: TATCGACGGTCATCGTCAGGT
R: TTGATGCACTCGACCAGCAAG		392
phzHF: GATTCCATCACAGGCTCG
R: CTAGCAATGGCACTAATCG		1752
lasAF: TGTCCAGCAATTCTCTTGC
R: CGTTTTCCACGGTGACC		1075
pvdAF: GCCAAGGTTTGTTGTCGG
R: CGCATTGACGATATGGAAC		1281
pilAF: ATGGAGAGCGGGATCGACAG
R: ATGCGGGTTTCCATCGGCAG		1675
pilBF: TCGCCATGACCGATACGCTC
R: ACAACCTGAGCCAGCCTTCC		408