Rhein Inhibits the Progression of Chemoresistant Lung Cancer Cell Lines via the Stat3/Snail/MMP2/MMP9 Pathway

Figure 4

Rhein regulated expression of MMP2, MMP9, Snail, and Stat3. A549, A549/Taxol, PC9, and PC9/GD cells were stimulated with rhein (0 μg/ml or 25 μg/ml or 50 μg/ml) for 24 h. (a) The mRNA levels of MMP2, MMP9, and Snail in A549, A549/Taxol, PC9, and PC9/GD cells were detected by real-time PCR. (b) The levels of MMP2, MMP9, Snail, and Stat3 and phosphorylation of Stat3 in A549, A549/Taxol, PC9, and PC9/GD cells were detected by western blotting. .

(a)

(b)