Antioxidant Activity and Inhibitory Effects of Black Rice Leaf on the Proliferation of Human Carcinoma Cells

Table 3

Antiproliferative activity of ethyl acetate subfractions derived from black rice leaf against several human cancer cell lines.

Cell lines	IC50 (μg/mL)
	Sub1	Sub2	Sub3	Sub4	Sub5	5-FU
HepG2
24 h	>400	>400	>400	>400	^a	^b
48 h	^a	>400	>400	^b	^c	^d
72 h	^a	>400	^b	^c	^d	^e
MCF-7
24 h	>400	>400	^a	>400	^c	^b
48 h	>400	>400	^a	^b	^d	^c
72 h	^a	^b	^c	^d	^e	^f
Caco-2
24 h	>400	>400	>400	>400	^a	^b
48 h	>400	>400	^a	>400	^b	^c
72 h	>400	>400	^b	^a	^c	^d

IC₅₀: concentration of the sample with 50% inhibition of cancer cells. The values of IC₅₀ are displayed as (). 5-FU, fluorouracil, is the reference drug. The letters ^a-e in the same row represent significant differences ().