Regulatory Role of B Cells and Its Subsets in Hepatitis E Virus Infection

Figure 7

Flow cytometric analysis showing the effect of IL-10/IL-10R blocking on HEV-rORF2p specific CD4 and CD8 T cells responses. PBMCs from 46 acute hepatitis E patients, 23 hepatitis E recovered individuals, and 54 healthy controls stimulated in the presence/absence of HEV-rORF2p, were cultured with/without IL-10/IL-10R blocking antibodies in the presence of IL-2. On tenth day, the cells were intracellularly stained to determine expression of IFN-γ on CD4⁺ T cells (%CD3⁺CD4⁺IFN-γ⁺) and CD8⁺ T cells (%CD3⁺CD8⁺IFN-γ⁺). Frequencies of CD3⁺CD4⁺IFN-γ⁺ T cells in (a) acute hepatitis E patients, (b) hepatitis E recovered individuals, (c) healthy controls and CD3⁺CD8⁺IFN-γ⁺ T cells in (d) acute hepatitis E patients, (e) hepatitis E recovered individuals, (f) healthy controls were assessed pre vs. post IL-10/IL-10R blocking. Pair wise comparisons were done on matched samples wherein dots represent individual values.