[Retracted] Neuroglobin Is Involved in the Hypoxic Stress Response in the Brain

Figure 1

Expression of neuroglobin protein in cortical neural stem cells during neocortical development. (a) Ngb-positive cells (red) were prominently distributed in the cytoplasm of IZ and VZ at E16. (b) Costaining of Ngb (red) with Sox2 (green) demonstrating Ngb colocalized with Sox2 in the cytoplasm of SVZ at E16, supported a potential role of Ngb in NSC migration, proliferation, and neurogenesis. (c) At E18, NSCs were mainly identified in IZ and CP, and Ngb was expressed in almost all NSCs. (d) NSCs increased at P3, and almost NSCs expressed Ngb. (e) At P7, the NSCs continued to migrate upwards widely distributed in the neocortex and nearly half of the NSCs displayed lower or undetectable level of Ngb protein. (f) the NSCs showed little or no Ngb immunostaining at P30. (g–i) Ngb and Sox2 double labeling showed Ngb-positive neural stem cells in CP at E18. (j–l) Ngb-immunoreactive cells were barely observed in the NSCs at P30 in the cortical V-VI layers. in (a, b); in (c–f); in (g–l).

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