Isolation, Characterization, and Effect on Biofilm Formation of Bacteriocin Produced by Lactococcus lactis F01 Isolated from Cyprinus carpio and Application for Biopreservation of Fish Sausage

Table 2

Evolution of activity and protein concentration following purification stages.

Purification stage	Volume (ml)	Activity (AU/ml)	Total activity (AU/ml)	Protein (μg/ml)	Specific activity (AU/μg)	Purification factor	Yield (%)
CFS	200	800	160000	25,7	31,12	1	100
PSA	20	3200	64000	86,8	37,82	1,18	40
PCM	10	6400	64000	169	37,86	1,08	40

CFS: crude culture supernatant; PSA: ammonium sulfate precipitate; PCM: chloroform-methanol precipitates.