BioMed Research International

Research Article

Isolation, Characterization, and Effect on Biofilm Formation of Bacteriocin Produced by Lactococcus lactis F01 Isolated from Cyprinus carpio and Application for Biopreservation of Fish Sausage

Table 3

Antimicrobial spectrum of C4B.


Indicator strains	Sources	Culture condition	Activity of C4B
Indicator strains	Sources	Culture condition	NCFS	PSA

Lactic acid bacteria
Lb sp. 2B.	Laboratory isolate	MRS. 37°C	+	+
Lentilactobacillus senioris	Strain	MRS. 37°C	+++	+++
Lb sp. M6p.	Laboratory isolate	MRS. 37°C	+++	+++
Lb sp. Til2MB.	Laboratory isolate	MRS. 37°C	+++	+++
Pathogenic Gram+ bacteria
Staphylococcus sp.	Clinical strain	BHI. 37°C	++	+++
Bacillus subtilis	ATCC13453	BHI. 37°C	++	++
Bacillus sp.	Clinical strain	BHI. 37°C	++	++
Staphylococcus sp. 2B.	Laboratory isolate	BHI. 37°C	++	+++
Staphylococcus sp. 1B.	Laboratory isolate	BHI. 37°C	++	++
Clostridium sp.	Clinical isolate	BHI. 37°C	++	++++
Pathogenic Gram− bacteria
Salmonella Typhimurium	ATCC 14028	BHI. 37°C	++	+++
Pseudomonas aeruginosa	ATCC 15692	BHI. 37°C	++	+++
Klebsiella pneumonae	ATCC 35595	BHI. 37°C	++	+++
Pseudomonas sp.	Clinical isolate	BHI. 37°C	++	++++
Pseudomonas sp.	Clinical isolate	BHI. 37°C	++	+++
Escherichia (SMR) sp.	Clinical isolate	BHI. 37°C	++	++
Shigella sp.	Clinical isolate	BHI. 37°C	++	+++
Escherichia sp. 2B.	Laboratory isolate	BHI. 37°C	++	+++
Escherichia sp. 3B.	Laboratory isolate	BHI. 37°C	++	+++
Serratia sp.	Clinical isolate	BHI. 37°C	—	—
Yessinia sp.	Clinical isolate	BHI. 37°C	++	++++
Salmonella sp. 3B.	Laboratory isolate	BHI. 37°C	++	+++
Vibrio sp. 2DS.	Laboratory isolate	BHI. 37°C	++	+++
Vibrio sp. 1DS.	Laboratory isolate	BHI. 37°C	++	+++
Salmonella sp. 2B.	Laboratory isolate	BHI. 37°C	++	+++
Pseudomonas sp. Z.	Clinical isolate	BHI. 37°C	++	+++

PSA: ammonium sulfate precipitate; NCFS: neutralized cell-free supernatant;–: no inhibition; +: 1.0–3.0 mm (weak); ++: 3.1–6.0 mm (good); +++: 6.1–14.0 mm (very good); ++++: >14.0 mm (strong).