Isolation, Characterization, and Effect on Biofilm Formation of Bacteriocin Produced by Lactococcus lactis F01 Isolated from Cyprinus carpio and Application for Biopreservation of Fish Sausage
Table 5
Effect of organic solvents, detergents, and surfactants on bacteriocin activity.
Activity (mm)
Activity (UA/ml)
Residual activity (%)
Effect of organic solvents
Control
8
800
100
Acetone
8
800
100
Chloroform
8
800
100
Methanol
8
800
100
Ethanol
8
800
100
Effect of detergents and surfactants
Control
10
800
100
SDS
7
400
70
Urée
10
800
100
Tween 20
10
800
100
Tween 80
10
800
100
Triton X-100
10
800
100
Effect of EDTA
Control
8
800
100
1%
10
800
125
2%
13
1600
162.5
3%
14
1600
175
4%
14
1600
175
5%
16
3200
200
Effect of NaCl
Control
8
800
100
1%
10
800
125
2%
10
800
125
3%
11
800
137.5
4%
12
1600
150
5%
12
1600
150
6%
13
1600
162.5
7%
13
1600
162.5
The positive control consists of an untreated solution of bacteriocin C4B. Residual activity is the percentage of activity in mm after treatment over the activity in mm of the control.
