Bushen Huoxue Formula Modulates Autophagic Flux and Inhibits Apoptosis to Protect Nucleus Pulposus Cells by Restoring the AMPK/SIRT1 Pathway

Figure 1

Positive effect of BSHXF on the viability of NP cells. (a) The identification about BSHXF components. (b–c) The identification of NP cells with CD24. (d) The impact of the BSHXF (5%, 10%, 15%, 20%, and 25%) on the NP cells was detected by a CCK-8 assay. (e) The proliferation of NP cells treated with various concentrations of serum without BSHXF was measured by CCK-8 assay. (f) The effect of BSHXF was detected using the CCK-8 assay after the cells were induced by TNF-α. (g–j) Western blotting was applied to determine the positive effect of BSHXF on NP cells. The group treated with serum with or without BSHXF; and ; the group treated with TNF-α, ^# and ^##.

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)