Bushen Huoxue Formula Modulates Autophagic Flux and Inhibits Apoptosis to Protect Nucleus Pulposus Cells by Restoring the AMPK/SIRT1 Pathway

Figure 4

BSHXF reinforces the effect of autophagy in NP cells. (a–d) Western blotting was applied to measure the level of LC3, beclin-1, and p62. NP cells were intervened in the presence of TNF-α and BSHXF. (e) Electron microscopy images of autophagosomes. NP cells were fostered with TNF-α and BSHXF. The group treated with serum containing BSHXF, and ; the TNF-α group, ^# and ^##.

(a)

(b)

(c)

(d)

(e)