[Retracted] Adropin Inhibits Vascular Smooth Muscle Cell Osteogenic Differentiation to Alleviate Vascular Calcification via the JAK2/STAT3 Signaling Pathway

Figure 1

Adropin is expressed at low levels in calcific vascular tissues and osteogenic differentiated VSMCs. (a, b) The protein expression of adropin in rat vascular tissues and VSMCs was quantified using western blotting. GAPDH was used as the loading control. (c) The mRNA expression levels of adropin in VSMCs were quantified using reverse-transcription quantitative PCR. GAPDH was used as the loading control. (d, e) The expression of adropin in VSMCs was evaluated using immunofluorescence. Each experiment was performed three times, and data are presented as the . VSMCs: vascular smooth muscle cells. ^#p <0.05 compared to vehicle.

(a)

(b)

(c)

(d)

(e)