[Retracted] Adropin Inhibits Vascular Smooth Muscle Cell Osteogenic Differentiation to Alleviate Vascular Calcification via the JAK2/STAT3 Signaling Pathway

Figure 6

Adropin inhibits osteogenic differentiation of VSMCs via the JAK2/STAT3 signaling pathway in vitro. (a) The mRNA expression levels of calcification-associated markers (BMP2, ALP, OPG, OCN, and Runx2) in VSMCs were quantified using reverse-transcription quantitative PCR. GAPDH was used as the loading control. (b) The ALP activity of VSMCs. GAPDH was used as the loading control. (c) The protein expression levels of calcification-associated markers (BMP2, ALP, OPG, OCN, and Runx2) in VSMCs were quantified using western blotting. GAPDH was used as the loading control. Each experiment was performed three times, and data are presented as the . VSMCs: vascular smooth muscle cells; JAK2: tyrosine protein kinase JAK2; STAT3: signal transducer and activator of transcription 3; BMP2: bone morphogenetic protein 2; ALP: alkaline phosphatase; OPG: osteoprotegerin; OCN: osteocalcin; Runx2: runt-related transcription factor 2. compared to control. ^# compared to the Cacl₂ group.

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