Research Article
Identification and Validation of Common Reference Genes for Normalization of Esophageal Squamous Cell Carcinoma Gene Expression Profiles
Table 1
Evaluation of the expression stability of the eight candidate internal reference genes by NormFinder.
| Rank number | Cancer esophageal tissues | Normal esophageal tissues | All esophageal tissues | Gene in ranking order | Stability value | Gene in ranking order | Stability value | Gene in ranking order | Stability value |
| 1 | PPIA | 0.51 | RPS18 | 0.65 | GAPDH | 0.15 | 2 | RPS18 | 0.64 | PPIA | 0.84 | PPIA | 0.19 | 3 | ACTB | 0.77 | B2M | 1.00 | ACTB | 0.25 | 4 | PGK1 | 0.94 | HPRT1 | 1.31 | B2M | 0.4 | 5 | GAPDH | 0.96 | GAPDH | 1.40 | HPRT1 | 0.56 | 6 | B2M | 1.13 | ATCB | 1.82 | RPS18 | 0.74 | 7 | HPRT1 | 1.17 | GUSB | 2.17 | GUSB | 0.87 | 8 | GUSB | 1.54 | PGK1 | 3.23 | PGK1 | 1.03 |
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Note: The lower the SV, the higher the stability.
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