Identification and Validation of Common Reference Genes for Normalization of Esophageal Squamous Cell Carcinoma Gene Expression Profiles

Table 2

Evaluation of the expression stability of the eight candidate internal reference genes by BestKeeper.

Group	Stability ranking	Gene name	Std dev [±CP]
Cancer esophageal tissues	1	PPIA	0.98
	2	RPS18	0.99
	3	ATCB	1.00
	4	PGK1	1.04
	5	GUSB	1.09
	6	B2M	1.17
	7	HPRT1	1.29
	8	GAPDH	1.42
Normal esophageal tissues	1	PPIA	0.77
	2	RPS18	0.78
	3	B2M	0.81
	4	HPRT1	1.12
	5	GAPDH	1.37
	6	ACTB	1.58
	7	GUSB	1.77
	8	PGK1	2.55
All esophageal samples	1	RPS18	0.89
	2	PPIA	1.09
	3	B2M	1.37
	4	ATCB	1.38
	5	GUSB	1.44
	6	GAPDH	1.54
	7	HPRT1	1.61
	8	PGK1	2.43