The Oxidative Drug Combination for Suppressing KRAS G12D Inducible Tumour Growth

Figure 3

Induction of apoptosis by ATO and D-VC combination in AK 192 KRAS mutant cancer cells. (a) The bright field images of cells after treatment with PBS (GM control), 1 mM D-VC, 5 μM ATO, combination of 1 mM DVC and 5 μM ATO and 1 mM DVC and 5 uM ATO and 5 mM NAC. Images taken at 24 hours top row and 48 h bottom row. Scale bar 50μM. (b) Total cell number of AK 192 cells under different conditions over time. (c) The graphical presentation of the PI positive cell population shown. (d) The apoptotic assay using Annexin-V and PI staining and flow cytometric analysis. The dot plots of the cells described in (a), gate was set using unstained sample.

(a)

(b)

(c)

(d)