The Oxidative Drug Combination for Suppressing KRAS G12D Inducible Tumour Growth

Figure 6

Histological analysis. The hematoxylin and eosin stained tissue, . (a) Histological analysis of tumour xenografts from immune-deficient NOD Scid mice with and without ATO/D-VC treatment. Untreated (PBS control) are as follows: (1) glandular structures with papillary hyperplasia of the epithelium, (2) inflammatory infiltration, and (3) oedema of the stroma. Treated ATO/D-VC: 1- necrosis. 2- subtotal necrosis. 3 - subtotal necrosis. (b) Histological analysis of tumour xenografts of immune-deficient Nu mice with and without ATO/D-VC treatment. Untreated (PBS control): 1- Tumour invasion into the capsule. 2 - The glands are separated by proliferating connective tissue. Treated ATO/D-VC: 1 - sclerosis and lipomatosis of the tumour. 2 - tumour necrosis. 3 - Decrease in growth index. (c) Histological analysis of organs of NOD Scid xenograft mice with and without ATO/D-VC treatment. Untreated (PBS control): 1 - Liver. The hepatic lobules retain a beam structure. Moderate Kupffer cell hyperplasia. 2 - Lungs. Moderate swelling of the interalveolar septa. Bronchi: the focal proliferation of the epithelium, partial desquamation, secretory active epithelium. Treated ATO/D-VC are as follows: (1) liver: Irregular hyperplasia of Kupffer cells. Small foci of extramedullary hematopoiesis are found in apoptotic cells. Fragmentation of the nucleus, pycnosis of the nucleus. Cytolysis of individual hepatocytes is noted. In the subcapsular zones, areas with signs of vacuolar dystrophy of hepatocytes are determined in places. (2) Lungs.: Slight focal alveolar oedema. (d) Histological analysis of organs of Nu xenograft mice with and without ATO/D-VC treatment. Untreated (PBS control) is as follows: (1) liver: moderate hyperplasia of single Kupffer cells. Cytolysis of single hepatocytes. Periportally moderate oedema in the liver. (2) Lungs: single perivascular lymphocytic infiltrates. Treated ATO/D-VC is as follows: (1) liver: extramedullary hematopoiesis. (blast cells). Moderate hyperplasia of Kupffer cells, cytolysis of certain groups of hepatocytes. (2) Lungs: single perivascular lymphocytic infiltrates.

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