LINC02273 Promotes Hepatocellular Carcinoma Progression via Retaining β-Catenin in the Nucleus to Augment Wnt Signaling

Figure 2

LINC02273 is a nuclear lncRNA and KD of LINC02273 inhibited proliferation of HCC cells. (a) The ratio of LINC02273, U6, and GAPDH was determined in the fractioned cytoplasmic and nuclear RNA of Hep3B and MHCC97H cells, with U6 and GAPDH as endogenous nuclear and cytoplasmic indicators, respectively. (b) qRT-PCR was performed to determine the KD of LINC02273 in Hep3B and MHCC97H cells. (c–e) MTT, colony formation, and sphere formation was conducted to determine the effects of LINC02273 KD on proliferation, colony formation, and sphere formation abilities. For multiple group comparison, we performed ANOVA analysis followed by Dunnett’s test. , .

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