Research Article
LINC02273 Promotes Hepatocellular Carcinoma Progression via Retaining β-Catenin in the Nucleus to Augment Wnt Signaling
Figure 5
LINC02273 knockdown led to decreased nuclear β-catenin accumulation and suppressed downstream gene expression. (a) β-Catenin transcription activity was determined by the TOP/FOP reporter system, with the signal of each cell line normalized to the corresponding NT group. (b) WB of β-catenin and p84 in fractioned nuclear protein, with p84 as the loading control. (c) The expression of β-catenin downstream genes (c-Myc, Cyclin D1, Survivin, MMP-7, and COX-2) was determined by qRT-PCR and normalized to the corresponding NT group. , .
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