Research Article
1,25-Dihydroxy Vitamin D3 Facilitates the M2 Polarization and β-Amyloid Uptake by Human Microglia in a TREM2-Dependent Manner
Figure 1
1,25(OH)2D3 downregulates Aβ-induced M1 cytokine expression, whilst upregulating M2 cytokine expression in HMO6 cells. Cells were incubated in DMEM exposed to 100 nM Aβ1-42 and treated with or without 1 μM of 1,25(OH)2D3 for 6 h or 12 h. mRNA was extracted, and expression of proinflammatory cytokines TNF-α (a), IL-1β (b), and IL-6 (c) or anti-inflammatory cytokines IL-4 (d), IL-10 (e), and CCL17 (f) was measured by quantitative real-time PCR. and vs. 0 h control group; # and ## vs. Aβ1-42-treated group.
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