| | In silico predicted parameters | Used servers and web addresses | L. donovani HASPB1 |
| | Antigenicity | VaxiJen v2.0, http://www.ddg-pharmfac.net/vaxijen/ | 1.4409 | | Allergenicity | AlgPred v2.0, https://webs.iiitd.edu.in/raghava/algpred2/ | Yes | | Protein solubility | Protein-sol, https://protein-sol.manchester.ac.uk/ | 0.749 (highly soluble) | | Number of residues | ExPASy ProtParam, https://web.expasy.org/protparam/ | 401 | | Molecular weight | 42196.62 | | pI | 4.64 | | Positively charged residues (Arg+Lys) | 53 | | Negatively charged residues (Asp + Glu) | 106 | | Half-life in mammalian reticulocytes | 30 hours | | Instability index | 21.34 (stable) | | Aliphatic index | 5.89 | | GRAVY score | -2.322 (highly hydrophilic) | | Signal peptide | SignalP-6.0, https://services.healthtech.dtu.dk/service.php?SignalP-6.0 | No signal peptide (sec/SPI) | | Transmembrane domain | DeepTMHMM, https://dtu.biolib.com/DeepTMHMM | No (most likely globular) | | Subcellular localization | DeepLoc2.0, https://services.healthtech.dtu.dk/service.php?DeepLoc-2.0 | Cell membrane (0.63) | | N-Glycosylation | NetNGlyc-1.0, https://services.healthtech.dtu.dk/service.php?NetNGlyc-1.0 | Single site at position 44 | | O-Glycosylation | NetOGlyc-4.0, https://services.healthtech.dtu.dk/service.php?NetOGlyc-4.0 | 16 sites at positions 3, 4, 6, 9, 23, 24, 32, 39, 41, 46, 96, 110, 124, 180, 320, and 334 | | Palmitoylation | CSS-palm, http://csspalm.biocuckoo.org/ | Single site at position 5 | | Serine (S), threonine (T), and tyrosine (Y) phosphorylation | NetPhos-3.1, https://services.healthtech.dtu.dk/service.php?NetPhos-3.1 | 28 sites at positions: 4, 9, and 36 (S); 6, 23, 32, 54, 68, 82, 96, 110, 124, 138, 152, 166, 180, 194, 208, 222, 236, 250, 264, 278, 292, 306, 320, 334, and 348 (T) | | Lysine acetylation | GPS-PAIL, http://pail.biocuckoo.org/ | 11 sites at positions 7, 11, 15, 22, 63, 77, 91, 105, 119, 133, and 147 | | Secondary structure | NetSurfP-3.0, https://services.healthtech.dtu.dk/service.php?NetSurfP-3.0 | Exposed residues in most sites, coils are frequent with a high probability of disordered regions | | Tertiary structure (3D model) | I-TASSER, https://zhanggroup.org/I-TASSER/ | -score: -0.79, estimated TM-score: , estimated root-mean-square deviation (RMSD): Å |
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