In Silico Characterization of an Important Metacyclogenesis Marker in Leishmania donovani, HASPB1, as a Potential Vaccine Candidate

Table 3

Human helper T-lymphocyte (HTL) epitope prediction for LdHASPB1 using the IEDB HLA reference set, with subsequent screening regarding antigenicity, allergenicity, and IFN-γ induction.

Allele	HTL epitope	Method	Percentile rank	Antigenicity	Allergenicity	IFN- inducer
HLA-DQA105 : 01/DQB103 : 01	TEANHGGATGVPPK	Consensus (comb.lib./smm/nn)	2.00	1.1249	No	Negative
HLA-DQA105 : 01/DQB103 : 01	TTEANHGGATGVPP	Consensus (comb.lib./smm/nn)	2.1	1.1017	No	Negative
HLA-DQA105 : 01/DQB103 : 01	TEANHGGATGVPP	Consensus (comb.lib./smm/nn)	1.6	1.0977	No	Negative
HLA-DQA105 : 01/DQB103 : 01	KTTEANHGGATGVPP	Consensus (comb.lib./smm/nn)	2.6	0.9802	No	Negative
HLA-DQA105 : 01/DQB103 : 01	TEANHGGATGVPPKH	Consensus (comb.lib./smm/nn)	2.6	1.1819	Yes	Negative
HLA-DQA105 : 01/DQB103 : 01	TTEANHGGATGVPPK	Consensus (comb.lib./smm/nn)	2.6	1.1256	Yes	Negative
HLA-DQA105 : 01/DQB103 : 01	ANHGGATGVPPKHT	Consensus (comb.lib./smm/nn)	2.5	0.7527	Yes	Negative
HLA-DQA105 : 01/DQB103 : 01	EANHGGATGVPPKHT	Consensus (comb.lib./smm/nn)	2.8	0.9030	No	Positive
HLA-DQA105 : 01/DQB103 : 01	EANHGGATGVPPKH	Consensus (comb.lib./smm/nn)	2.9	1.0807	No	Negative
HLA-DQA105 : 01/DQB103 : 01	TEANHGGATGVPPKHT	Consensus (comb.lib./smm/nn)	2.7	1.0112	No	Positive