In Silico Characterization of an Important Metacyclogenesis Marker in Leishmania donovani, HASPB1, as a Potential Vaccine Candidate
Table 4
Human cytotoxic T-lymphocyte (CTL) epitope prediction for LdHASPB1 using the IEDB HLA reference set, with subsequent screening in terms of antigenicity and allergenicity.
HLA allele
Epitope length
Sequence
Percentile rank: <1
Antigenicity
AllerTOP allergenicity
HLA-B07 : 02
10
VPPKHTGSAM
0.04
0.2065
Yes
HLA-A68 : 01
9
DSAKEPQKR
0.2
1.3388
Yes
HLA-B07 : 02
9
PPKHTGSAM
0.18
0.3758
No
HLA-A11 : 01
9
SAMNDSAPK
0.25
0.7898
Yes
HLA-A33 : 01
9
DSAKEPQKR
0.26
1.3388
Yes
HLA-B07 : 02
9
VPPKHTGSA
0.35
0.1872
Yes
HLA-A68 : 01
9
SAMNDSAPK
0.97
0.7898
Yes
HLA-A68 : 02
10
EANHGGATGV
0.34
1.1771
No
HLA-A11 : 01
10
GSAMNDSAPK
0.52
1.3978
Yes
HLA-A11 : 01
9
GGATGVPPK
0.53
0.5969
Yes
HLA-A30 : 01
9
SAMNDSAPK
0.52
0.7898
Yes
HLA-B51 : 01
9
EPQKRADNI
0.56
0.7975
No
HLA-A30 : 01
9
GGATGVPPK
0.56
0.5969
Yes
HLA-B44 : 03
10
QEKNEDGHNV
0.49
1.1248
Yes
HLA-B44 : 02
10
QEKNEDGHNV
0.44
1.1248
Yes
HLA-B35 : 01
10
VPPKHTGSAM
0.6
0.2065
Yes
HLA-A30 : 01
9
SAKEPQKRA
0.75
1.5432
No
HLA-B07 : 02
9
APKEDGHTQ
0.7
1.5982
No
HLA-B08 : 01
9
EPQKRADNI
0.67
0.7975
No
The final qualified epitopes regarding percentile rank, antigenicity, and allergenicity.
