In Silico Characterization of an Important Metacyclogenesis Marker in Leishmania donovani, HASPB1, as a Potential Vaccine Candidate
Table 5
Dog leukocyte antigen (DLA) class-I epitope prediction for LdHASPB1 using the IEDB server, with subsequent screening in terms of antigenicity and allergenicity.
DLA allele
Position
Sequence
Percentile rank
Antigenicity
AllerTOP allergenicity
DLA-8803401
33-42
GVPPKHTGSA
11
0.2291
Yes
3-12
QQNDGDAQEK
11
1.2084
Yes
7-16
KDSAKEPQKR
12
1.5395
No
34-43
VPPKHTGSAM
13
0.2065
Yes
29-38
GGATGVPPKH
14
0.7968
Yes
DLA-8850101
33-42
GVPPKHTGSA
6.9
0.2291
Yes
34-43
VPPKHTGSAM
11
0.2065
Yes
3-12
QQNDGDAQEK
14
1.2084
Yes
25-34
EANHGGATGV
17
1.1771
No
22-31
KTTEANHGGA
21
1.0364
No
DLA-8850801
3-12
QQNDGDAQEK
1.3
1.2084
Yes
7-16
KDSAKEPQKR
2.8
1.5395
No
48-57
TQKNDGDGPK
5.5
0.7858
No
34-43
TQKNDGDGPK
5.5
0.7858
No
20-29
TQKNDGDGPK
5.5
0.7858
No
The final qualified epitopes regarding percentile rank, antigenicity, and allergenicity.
