Transcriptomic Network Regulation of Rat Tooth Germ from Bell Differentiation Stage to Secretory Stage: MAPK Signaling Pathway Is Crucial to Extracellular Matrix Remodeling

Figure 2

mRNA differential expression analysis. (a) DEGs clustering heatmap (top 50). Each column represents different samples, and the lines represent different genes. Red indicates that the gene was highly expressed in the sample, and blue indicates that the gene was lowly expressed in the sample. As the difference is greater, the color is deeper. (b) Red dots represented upregulated genes, purple dots represented downregulated genes, and gray dots represented genes with no significant change. The -axis was -log10 ( value), and the bigger quantity reflects a lesser difference (only the data with a value < 0.05 are shown). (c) Every ten most significantly enriched GO terms were selected from the three categories of BP (purple), CC (orange), and MF (yellow), displayed in the figure. If there were less than 10 GO terms, they were all displayed. (d) -axis is gene ratio, indicating the ratio of differentially expressed mRNAs under the pathway entry to the total number of DEGs. The larger gene ratio value reflects a higher enrichment of DEGs in the KEGG pathway. The bubble size indicates the number of enriched DEGs, and the color represents the value magnitude. (e) Clustering results of differentially expressed genes. (f) PPI network of the biggest clusters (minimum required interaction score is high confidence): only the included proteins were found to correspond to other proteins.

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