[Retracted] Long Noncoding RNA LINC01503 Silencing Suppresses KLK4 Expression to Impede Pancreatic Cancer Development as miR-1321 Sponge

Figure 4

LINC01503 competed with KLK4 to sequester miR-1321. (a) Three candidate miRNAs possibly interacting with LINC01503 were predicted by DIANA and starBase databases. (b) qRT-PCR examined the expression of candidate miRNAs upon LINC01503 knockdown. (c) The binding sites of LINC01503 on miR-1321 were shown, and luciferase reporter assay evaluated the combination of LINC01503 and miR-1321. (d) RIP assay detected the enrichment of LINC01503 and miR-1321 in anti-Ago2. (e) The TargetScan and miRDB databases predicted the binding affinity and binding sequence of miR-1321 to KLK4. (f) Western blot examined KLK4 protein level in PC cells and normal pancreatic cells. (g) Luciferase reporter assay examined the combination of miR-1321 and KLK4. (h) Effects of miR-1321 upregulation or inhibition on KLK4 expression were detected through qRT-PCR. (i, j) qRT-PCR and western blot assays monitored KLK4 mRNA and protein levels in PC cells upon indicated transfections. , , and .

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