Circular RNA circ_0026218 Suppressed Atherosclerosis Progression via miR-338-3p/SIRT6 Axis

Figure 4

SIRT6 is a downstream target of miR-338-3p. (a) The TargetScan database was exploited to predict the binding sequence between miR-338-3p and SIRT6 3-UTR. (b) The predicted binding relations were testified by dual-luciferase reporter gene assay. (c–f) SIRT6 mRNA expressions and protein levels in HUVECs treated with discrepant concentrations (0 μg/mL, 50 μg/mL, 75 μg/mL, or 100 μg/mL) of ox-LDL for 24 h or 100 μg/mL ox-LDL for varied time points (0 h, 24 h, 48 h, or 72 h) were exposed by qRT-PCR and Western blot. (g, h) SIRT6 mRNA and protein expression in HUVECs with miR-338-3p or circ_0026218 overexpression were detected by qRT-PCR and Western blot. , , .

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