Research Article
High Expression of SMO and GLI1 Genes with Poor Prognosis in Malignant Mesothelioma
Table 1
Retrotranscriptional response system and reaction conditions.
| The reverse transcription system | Reaction conditions |
| DNA digester buffer (2 ul) | 42°C | 2 min | gDNA digester (1 ul) | 42°C | 2 min | Template RNA (1 ul) | 42°C | 2 min | RNase-free H2O (6 ul) | 42°C | 2 min | 2XHifair II SuperMix plus (10 ul) | 25°C | 5 min | 42°C | 30 min | 85°C | 5 min |
|
|