SPRR3 Contributes to Aggressiveness of Pancreatic Cancer Cells via NF-κB Signaling Pathway

Figure 5

SPRR3 regulated the NF-κB signaling in pancreatic cancer. (a) NF-κB luciferase reporter assay showed that NF-κB transcription activity was dramatically elevated in SPRR3-upregulating cells, while reduced in SPRR3-silenced cells. (b) The levels of nuclear p65 in indicated cell lines. (c) The mRNA levels of NF-κB downstream genes. (d) NF-κB inhibitor inhibited colonies formed in soft agar of SPRR3-overexpressing cells. (e) NF-κB inhibitor inhibited BrdU positive cells of SPRR3-overexpressing cells. (f) NF-κB inhibitor inhibited invasive ability of SPRR3-overexpressing cells. .

(a)

(b)

(c)

(d)

(e)

(f)