Two Main Cancer Biomarkers as Molecular Targets of Binase Antitumor Activity

Figure 2

The immunofluorescent colocalization of binase with EGFR and RAS proteins. The colocalization of binase with EGFR protein was assessed in BT-20 cells (a, c) and with RAS protein in A549 cells (b, d). Panels (a) and (b) represent the overall image of the cells while panels (c) and (d) show the cross-sections of the individual cells. In the assay, cells were grown on glass coverslips, treated with binase (100 μg/mL) for 1 min, 15 min, 60 min, 24 h, and 48 h or left untreated (control), and fixed and stained with the primary mouse monoclonal anti-EGFR (sc-120) or mouse anti-pan Ras (sc-166691) and rabbit anti-binase antibodies followed by the secondary antibodies conjugated to Alexa Fluor 647 (for EGFR and pan Ras, red) and Alexa Fluor 555 (for binase, green). The cell nuclei were stained with DAPI.

(a)

(b)

(c)

(d)