Spectrum of Beta-Thalassemia Mutations in Potential Carriers with Microcytic Hypochromic Anemia from Mazandaran and Golestan, Northern Provinces of Iran

Table 2

Specific primers for PCR sequencing of HBB gene.

HBB gene	Specific primer pairs	Annealing Tm	PCR product sizes
Exon 1 and 2	F: 5 CTGAGGGTTTGAAGTCCAACTCC 3	60°C	808 bp
	R: 5 CTTCCACACTGATGCAATCATTC 3
Exon 3	F: 5 CAATGTATCATGCCTCTTTGCAC 3	60°C	580 bp
	R: 5 GCACTGACCTCCCACATTCC 3