Autism-Like Behavior and Epigenetic Changes Associated with Autism as Consequences of In Utero Exposure to Environmental Pollutants in a Mouse Model

Table 3

Differentially methylated regions and associated genes.

VPA			Manganese			Lead
Gene ID	CpG	VPA : Ctl	Gene ID	CpG	Mn : Ctl	Gene ID	CpG	Pb : Ctl
Col2a1	chr15		Atf1	chr15		Lpo	chr11
Glt25d2	chr1		Lrp5	chr19		Alox5	chr6
App	chr16		Ercc3	chr18		Arhgef10	chr8
Chd7	chr4		Pnpla3	chr15		Pkia	chr3
Ptprz1	chr6		Gpr120	chr19		Frmd4b	chr6
Nf2	chr11		Rasgrf2	chr13		Chd7	chr4
Rab3gap1	chr1		Chd7	chr4		Derl2	chr11
Nmnat2	chr1		Hivep2	chr10		Ctsf	chr19
Eif5a	chr11		Ctxn1	chr8		Bsn	chr9
Pi4k2a	chr19		Ovol1	chr19		Odz2	chr11
R3hdm1	chr1		Hk2	chr6		Rpp38	chr2
			Slc13a5	chr11		Mtx2	chr2
			Adcy1	chr11		Fads3	chr19
			Scn5a	chr9		Plxna1	chr6
			Hs3st3a1	chr11		Zbtb5	chr4
			Pi4k2a	chr19		Dguok	chr6
			Dmtf1	chr5		Dnm2	chr9
			Gdap1	chr1		Trib2	chr12
			Csmd1	chr8		Prpsap2	chr11
			Tmeff2	chr1		Dst	chr1
			Trib2	chr12
			Hat1	chr2
			Prmt8	chr6
			Mrps28	chr3
			Tnrc6b	chr15

CpG array probe names were associated with gene IDs (UCSC Genome Browser). All array data was normalized to the internal sheered standard and control data was compared to experimental data for differential methylation using ratios. Reported significant differences were defined by >2-fold change, -test (). The resulting changes in methylation for exposures were 11 for VPA (5 down, 6 up), 25 for Mn (7 down, 18 up), and 20 for Pb (6 down, 14 up).