Sleep Deprivation Influences Circadian Gene Expression in the Lateral Habenula

Figure 2

The effect of LHb lesion on sleep-wake times following sleep deprivation in rats. (a) indicates the typical EEG and EMG recording, respectively, for wake, NREMS, and REMS times. The wake state was identified by the presence of desynchronized EEG and high EMG activity. The NREMS consisted of high-amplitude slow waves together with a low EMG relative to awake. The REMS was identified by the presence of regular θ activity coupled with low EMG relative to NREMS. (B1), (C1), and (D1), respectively, show the wake, NREMS, and REMS times following sleep deprivation before and after LHb lesion. Data from 18 h EEG recording were analyzed and presented as means ± SEM (). , , compared, respectively, difference between the wake, NREMS, and REMS times before and after sleep deprivation. , , compared, respectively, difference between the wake and NREMS times following deprivation before and after LHb lesion. (B2), (C2), and (D2) show, respectively, the graphs of wake, NREMS, and REMS plotted per 6 h for a 24 h period before and after lesion. compared with sleep deprivation at the same time point.

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