Neuroprotective Effect of DAHP via Antiapoptosis in Cerebral Ischemia

Figure 6

Protein expression levels for inflammation cytokines in the rat cortex in penumbral and stroke areas were quantified by Western blotting. (a, c, d) DAHP downregulated caspase-3, TNF-α, and iNOS protein expression. Ischemic reperfusion induced a substantial caspase-3, TNF-α, and iNOS expression in the vehicle treatment and MCAO groups (, versus the sham group), which was significantly attenuated by DAHP ( and , versus the DAHP-treated group). (b) DAHP increased Bcl-2 expression (, versus the DAHP-treated group). Total Bcl-2 expression was significantly increased 24 h after ischemic reperfusion in the DAHP group, relative to MCAO or vehicle treatment. (e) DAHP induced COX-2 protein expression. Ischemic reperfusion induced COX-2 expression in the MCAO- and DMSO-treated groups, but COX-2 expression was more upregulated in the DAHP group than in the MCAO and DMSO groups (, versus the DAHP-treated group). , versus the sham group (c–e).