Wild Bitter Melon Exerts Anti-Inflammatory Effects by Upregulating Injury-Attenuated CISD2 Expression following Spinal Cord Injury

Figure 3

Knockdown of CISD2 expression in neural cells significantly influenced mRNA expression levels in PPAR-β, indicating upstream regulation of CISD2 on PPAR-β (a). mRNA levels of NF-κB, COX-2, and RANTES were determined by semiquantitative RT-PCR in neural cells with or without si-CISD2 transfection. The results shown are from one of the experiments that were repeated for at least 3 times. Quantification of relative band intensity of semiquantitative RT-PCR showed that CISD2 knockdown in neural cells led to enhanced mRNA expression levels in NF-κB (b) and downstream proinflammatory mediators including COX-2 (c) and RANTES (d). (e) Results of real-time qRT-PCR for mRNA levels of PPAR-β in neural cells with or without si-CISD2 transfection. Vertical bars indicate the (SEM) of mRNA expression (). and indicate a statistically significant difference between the control and si-CISD2 groups using independent two-sample -tests.

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