The Effects of Poria cocos on Rho Signaling-Induced Regulation of Mobility and F-Actin Aggregation in MK-801-Treated B35 and C6 Cells

Figure 2

Effects of PRP and WP on actin filament reorganization in B35 and C6 cells treated with MK-801. MK-801-treated B35 and C6 cells on coverslips were incubated with PRP or WP accordingly. ActinGreen™ 488 RreadyProbes™ reagent was used for F-actin in staining in B35 and C6 cells. (a) Red arrows show actin nucleation and blue arrows show F-actin condensation. The numbers of (b) actin nucleation and (c) F-actin condensation were obtained by counting the condensed actin spot in nuclei or condensed actin filament in extended-cytoplasm in 100 cells under different fields of view. The bar charts were made from three independent batches of experiments and analyzed by using Student’s -test ( value <0.05; value <0.01) analysis.

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