The Effects of Poria cocos on Rho Signaling-Induced Regulation of Mobility and F-Actin Aggregation in MK-801-Treated B35 and C6 Cells

Figure 3

PRP and WP regulate MK-801-induced ROCK1, PFN1, and p-MLC2 regulation. Western blotting revealed the expression changes of ROCK1, PFN1, and p-MLC2 induced by PRP/WP in (a) B35 and (c) C6 cells treated with MK-801. The protein expression was quantified by using ImageJ software. Beta-actin was used as a normalization control to calculate relative expression of examined target. The bar chart was constructed according to the data of three independent western blot experiments that analyzed three different batches of protein extracts of drug-treated (b) B35 and (d) C6 cells. The data was analyzed by using Student’s -test ( value <0.05; value <0.01) analysis.

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