Liver X Receptor Agonists Inhibit the Phospholipid Regulatory Gene CTP: Phosphoethanolamine Cytidylyltransferase-Pcyt2
Figure 1
Inhibition of the CDP-ethanolamine
pathway by 25-hydroxycholesterol. Mouse cells C3H10T1/2 were treated with 10 ng/μL of 25-OH (filled triangles), “pulsed” with 14C-Etn
for 1 hour and “chased” with an excess of unlabelled ethanolamine as indicated.
14C-ethanolamine (A), 14C-phosphoethanolamine (B), and 14C-CDP-ethanolamine (C) products were determined from the water phase and the
radio-labeled PE (E) was quantified from
the organic phase. Untreated cells grown in serum-free media (open squares)
were used as controls. Data shown are from three independent experiments
performed in duplicate; (*) indicates differences between treatments at P < .05.
