Two Distantly Spaced Basic Patches in the Flexible Domain of Huntingtin-Interacting Protein 1 (HIP1) Are Essential for the Binding of Clathrin Light Chain

Figure 2

HIP1 468-530 subfragment in the opened region is stable only at low temperature. Equilibrium far UV CD scans were performed at 4^∘C (open triangle), 10^∘C (open diamond), 37^∘C (cross), and 83^∘C (open circle). The position of the 222 nm signal did not change with temperature, but the 208 nm signal shifted as the sample was heated. The 222/208 nm ratios were calculated from the raw CD data. Because the signal below 200 nm was noisy, we did not attempt to get the helix content from the CD data.