Dephosphorylation of Centrins by Protein Phosphatase 2C 𝛼 and 𝛽

Figure 3

Characterization of dephosphorylation of P-centrin1 by PP2C. Centrin1 (0.2 𝜇g) was phosphorylated by CK2 (0.2 𝜇g) and [𝛾−³²P]ATP. (a)–(d) Dephosphorylation by 0.08 𝜇g PP2C𝛽 performed in the presence of 1 mM Mg²⁺ unless indicated otherwise. (a) Protein dependence. (b) Requirement for Mg²⁺-ions for activity. (c) Inhibition by Ca²⁺-ions. (d) Stimulation by oleic acid. (e) Effect of PP2C isozymes 𝛼, 𝛽, and 𝛿 on P-Thr¹³⁸-centrin1.

(a)

(b)

(c)

(d)

(e)