Multiplex Detection and Genotyping of Point Mutations Involved in Charcot-Marie-Tooth Disease Using a Hairpin Microarray-Based Assay
Figure 1
Hybridization specificity. Each spot type on the microarray was presented in five vertical replicates. (a) Hybridization of amplified DNA from healthy individuals (WT). All WT probe types corresponding to hybridized targets were detected. (b) Hybridization of mutant sequence DNA (C42R). WT and MT probes for the heterozygous C42R mutation were clearly detected with a discrimination ratio of 2.2. All other WT spots corresponding to hybridized targets were also detected.
